Affinity Chromatography of Potato Lactate Dehydrogenase

NH,CI. None of the adaptations could be attributed to the high-protein diet per se since they were not evident in those rats fed a high-protein diet and given NaHC03 solution to drink. Of particular interest is the increased rate of gluconeogenesis found in kidney slicesfrom rats fed ona high-protein diet. Krebs et al. (1963) have observed an increased rate of renal gluconeogenesis in rats fed on a low-carbohydratediet. However, their lowcarbohydrate diet was enriched in protein and it seems that the increased rate of gluconeogenesis observed may be attributed, at least in part, to the acidogenic effects of the diet.